Hung S S, Moon T W, Hilton J W, Slinger S J
J Nutr. 1982 Aug;112(8):1590-9. doi: 10.1093/jn/112.8.1590.
The appearance of radioactivity after the oral administration of 3 microCi D-alpha-[5-methyl-3H]tocopherol and 10 microCi DL-alpha-[3',4'-14C]tocopheryl acetate in plasma, liver, kidney, spleen and heart of rainbow trout showed an exponential increase up to 32 hours, followed by a plateau or slight decline from 32 to 64 hours. Radioactivity in the skeletal muscle increased exponentially up to 8 hours followed by a slower liner increase up to 64 hours. Comparisons of plasma 3H and 14C radioactivity suggested that the uptake of D-alpha-tocopherol (EOH) was 6 to 18 times greater than DL-alpha-tocopheryl acetate (EAc) in the first 4 hours and 2 to 3 times greater between 8 and 64 hours. At the plateau, the amount of 3H and 14C radioactivity incorporated per unit wet weights of tissue decreased in the order liver greater than kidney greater than plasma greater than spleen greater than heart much greater than skeletal muscle. More than 87% of the 3H and 14C radioactivity after 16 hours was found to be free alpha-tocopherol in both plasma and liver. The radioactivity labeled vitamins were bound primarily to plasma low-density lipoprotein (density 1.015 to 1.085). These studies support the hypothesis that the uptake, transport and distribution fo EAc after hydrolysis in the gastrointestinal tract of trout follows a pattern similar to that of EOH.
在虹鳟鱼的血浆、肝脏、肾脏、脾脏和心脏中，口服3微居里D-α-[5-甲基-³H]生育酚和10微居里DL-α-[3',4'-¹⁴C]生育酚醋酸酯后放射性的出现显示，在32小时内呈指数增加，随后在32至64小时内达到平稳或略有下降。骨骼肌中的放射性在8小时内呈指数增加，随后在64小时内呈较慢的线性增加。血浆中³H和¹⁴C放射性的比较表明，在最初4小时内，D-α-生育酚（EOH）的摄取量比DL-α-生育酚醋酸酯（EAc）大6至18倍，在8至64小时之间大2至3倍。在平稳期，每单位组织湿重中掺入的³H和¹⁴C放射性量按肝脏＞肾脏＞血浆＞脾脏＞心脏＞骨骼肌的顺序下降。16小时后，在血浆和肝脏中发现超过87%的³H和¹⁴C放射性是游离α-生育酚。放射性标记的维生素主要与血浆低密度脂蛋白（密度1.015至1.085）结合。这些研究支持了这样的假设，即鳟鱼胃肠道中EAc水解后的摄取、运输和分布模式与EOH相似。
