Palmer M R, Sorensen S M, Freedman R, Olson L, Hoffer B, Seiger A
J Pharmacol Exp Ther. 1982 Aug;222(2):480-7.
The relative importance of extrinsic and intrinsic factors for the differential sensitivity of Purkinje neurons in long-sleep (LS) and short-sleep (SS) lines of mice was investigated using cerebellar grafts to the anterior chamber of the eye. Cerebellar anlagen from fetal LS and SS donors survive and mature in oculo when grafted within and across lines. Mature cerebellar transplants from all four groups contained Purkinje cells that show sustained spontaneous discharge; excitation and inhibition are readily evoked by electrical stimulation of the surface of the graft. Superfusion of ethanol into the anterior chamber depresses Purkinje cell discharge, but neurons from LS donors are approximately 1 order of magnitude more sensitive than SS neurons; this differential sensitivity is unaltered by the host recipient line used. These data strongly suggest that the differential sensitivity of Purkinje cells to ethanol in the LS and SS mouse lines is an intrinsic property of the cerebellum.
利用将小脑移植到眼房的方法，研究了外在因素和内在因素对长睡眠（LS）和短睡眠（SS）品系小鼠浦肯野神经元差异敏感性的相对重要性。当在品系内和品系间进行移植时，来自胎儿LS和SS供体的小脑原基在眼内存活并成熟。来自所有四组的成熟小脑移植组织都含有表现出持续自发放电的浦肯野细胞；通过对移植组织表面进行电刺激，很容易诱发兴奋和抑制。向前房灌注乙醇会抑制浦肯野细胞的放电，但来自LS供体的神经元比SS神经元的敏感性大约高1个数量级；这种差异敏感性不会因所用的宿主受体品系而改变。这些数据有力地表明，LS和SS小鼠品系中浦肯野细胞对乙醇的差异敏感性是小脑的一种内在特性。
