Pritzker K P, Renlund R C, Read D H, Cheng P T, Harrington D D
Am J Vet Res. 1982 Aug;43(8):1481-8.
The nature and histologic environment of birefringent crystals found incidentally at necropsy in the liver and adrenal glands of dogs fed a thiamine-deficient diet were studied. The crystals were identified as calcium stearate by ultrastructural and x-ray microdiffraction techniques. Crystals were observed intracellularly within cytoplasmic vacuoles and extracellularly within sinusoids. Generally, crystals were also observed in pair-fed controls that were given a purified diet (equal in weight to that consumed by the thiamine-deficient animals) plus supplemental thiamine. Crystal deposits were found in trace amounts in a few of the ad libitum-fed controls. Although thiamine deficiency may be involved in promoting crystal deposition in the adrenal cortex, calcium stearate crystal formation within the adrenal glands and the liver appears unrelated to dietary thiamine deficiency per se, but is probably related to deficiency of an unidentified nutrient in the purified thiamine-deficient diet.
对喂食硫胺素缺乏饮食的犬类肝脏和肾上腺尸检时偶然发现的双折射晶体的性质和组织学环境进行了研究。通过超微结构和X射线微衍射技术将晶体鉴定为硬脂酸钙。在细胞质空泡内的细胞内以及肝血窦内的细胞外均观察到晶体。通常，在成对喂食的对照动物中也观察到晶体，这些对照动物喂食纯化饮食（重量与硫胺素缺乏动物所消耗的饮食相等）并补充硫胺素。在一些随意进食的对照动物中发现了微量的晶体沉积物。尽管硫胺素缺乏可能参与促进肾上腺皮质中晶体的沉积，但肾上腺和肝脏内硬脂酸钙晶体的形成似乎与饮食中硫胺素缺乏本身无关，而可能与纯化的硫胺素缺乏饮食中一种未确定营养素的缺乏有关。
