Barsukov L I, Kulikov V I, Simakova I M, Tikhonova G V, Ostrovskii D N, Bergelson L D
Eur J Biochem. 1978 Oct;90(2):331-6. doi: 10.1111/j.1432-1033.1978.tb12609.x.
Incubation of Micrococcus lysodeikticus protoplasts with phosphatidylcholine liposomes and rat liver exchange proteins (pH 5.1 supernatant fraction) resulted in replacement of about one half of the bacterial total phospholipids by phosphatidylcholine. Protoplasts modified by phosphatidylcholine showed a decreased rate of oxidation of exogenous substrates (NADH, malate) and decreased ferricyanide reductase activity as compared to the initial protoplasts. At the same time incorporation of phosphatidylcholine had no influence on the level of endogeneous respiration. Protoplasts modified by phosphatidylcholine were osmotically more stable than the initial protoplasts. After osmotic lysis of the phosphatidylcholine protoplasts their NADH (malate) oxidase and ferricyanide reductase activities were restored. Incorporation of phosphatidylcholine into membrane ghosts, obtained by osmotic rupture of the initial protoplasts had only small if any effect on the malate and NADH oxidase and dehydrogenase activities. It is concluded that phosphatidylcholine in incorporated predominantly into the outer part of cytoplasmic membrane and that proteinmediated transfer of phosphatidylcholine results in restoration of the permeability barrier due to repair of local defects in the initial protoplast membrane.
用磷脂酰胆碱脂质体和大鼠肝脏交换蛋白（pH 5.1上清液部分）孵育溶壁微球菌原生质体，结果约一半的细菌总磷脂被磷脂酰胆碱取代。与初始原生质体相比，经磷脂酰胆碱修饰的原生质体对外源底物（NADH、苹果酸）的氧化速率降低，铁氰化物还原酶活性降低。同时，磷脂酰胆碱的掺入对内源呼吸水平没有影响。经磷脂酰胆碱修饰的原生质体在渗透压方面比初始原生质体更稳定。磷脂酰胆碱原生质体经渗透裂解后，其NADH（苹果酸）氧化酶和铁氰化物还原酶活性得以恢复。将磷脂酰胆碱掺入通过初始原生质体渗透破裂获得的膜微粒中，对苹果酸和NADH氧化酶及脱氢酶活性即使有影响也很小。得出的结论是，磷脂酰胆碱主要掺入细胞质膜的外部，并且磷脂酰胆碱的蛋白质介导转移由于修复初始原生质体膜中的局部缺陷而导致渗透屏障的恢复。
