牛红细胞膜的血型糖蛋白。主要成分的分离及初步特性分析

Glycophorins of bovine erythrocyte membranes. Isolation and preliminary characterization of the major component.

作者信息

Murayama J, Tomita M, Hamada A

出版信息

J Biochem. 1982 Jun;91(6):1829-36. doi: 10.1093/oxfordjournals.jbchem.a133879.

DOI:10.1093/oxfordjournals.jbchem.a133879

Abstract

Crude glycophorin fraction was prepared from bovine erythrocyte membranes by extraction with lithium diiodosalicylate and partition in aqueous phenol. The crude fraction was further separated into three fractions by gel chromatography and ion-exchange chromatography. The major fraction, designated glycophorin BA, accounts for 80% of the crude fraction. Its carbohydrate content was 79% by weight including galactose, N-acetylgalactosamine, N-acetylglucosamine and N-glycolylneuraminic acid. Iodosobenzoate treatment of glycophorin BA produced two fragments; one is a highly glycosylated segment and the other a hydrophobic peptide. The amino-terminal sequence of the hydrophobic peptide representing the carboxyl-terminal half of glycophorin BA was determined. The other two fractions, IIIa and IIIb, were still heterogeneous when analyzed by gel electrophoresis. Fraction IIIa contained two glycophorins having apparent molecular weights of 46,000 and 42,000, while fraction IIIb contained a glycoprotein having a marked tendency to aggregate.

摘要

通过用二碘水杨酸锂提取并在苯酚水溶液中分配，从牛红细胞膜制备粗制血型糖蛋白组分。通过凝胶色谱法和离子交换色谱法将粗制组分进一步分离成三个组分。主要组分，称为血型糖蛋白BA，占粗制组分的80％。其碳水化合物含量按重量计为79％，包括半乳糖、N-乙酰半乳糖胺、N-乙酰葡糖胺和N-羟乙酰神经氨酸。对血型糖蛋白BA进行碘代苯甲酸处理产生两个片段；一个是高度糖基化的片段，另一个是疏水肽。确定了代表血型糖蛋白BA羧基末端一半的疏水肽的氨基末端序列。通过凝胶电泳分析时，另外两个组分IIIa和IIIb仍然是不均一的。组分IIIa包含两种表观分子量分别为46,000和42,000的血型糖蛋白，而组分IIIb包含一种具有明显聚集倾向的糖蛋白。