Möller A, Gabriels J E, Lafer E M, Nordheim A, Rich A, Stollar B D
J Biol Chem. 1982 Oct 25;257(20):12081-5.
Brominated poly(dG-dC) . poly(dG-dC), which forms a stable Z-DNA helix under physiological salt conditions, was used as an antigen to produce monoclonal antibodies from mice. The anti-Z-DNA antibodies were specific for the Z conformation as was shown by direct binding studies and a competitive solid phase assay. Competition could only be seen by brominated poly(dG-dC) . poly(dG-dC) under low and high salt conditions and unmodified poly(dG-dC) . poly(dG-dC) under high salt conditions. No binding could be seen to a number of other polynucleotides, including native and denatured DNA. Among the anti-Z-DNA monoclonal antibodies, examples differed in specificity: binding of one monoclonal antibody was blocked by methylation of cytosine; whereas the other antibody reacted equally well with the unmodified polymer or methyl cytosine-containing polymer in high ionic strength. The two antibodies are specific for different localized features of Z-DNA structure.
溴化聚（dG-dC）·聚（dG-dC）在生理盐条件下形成稳定的Z-DNA螺旋，被用作抗原从小鼠体内产生单克隆抗体。直接结合研究和竞争性固相分析表明，抗Z-DNA抗体对Z构象具有特异性。只有在低盐和高盐条件下的溴化聚（dG-dC）·聚（dG-dC）以及高盐条件下的未修饰聚（dG-dC）·聚（dG-dC）才能观察到竞争现象。对包括天然和变性DNA在内的许多其他多核苷酸均未观察到结合。在抗Z-DNA单克隆抗体中，实例的特异性有所不同：一种单克隆抗体的结合被胞嘧啶甲基化所阻断；而另一种抗体在高离子强度下与未修饰的聚合物或含甲基胞嘧啶的聚合物反应同样良好。这两种抗体对Z-DNA结构的不同局部特征具有特异性。
