Phillip M J, Molinari J A
Anticancer Res. 1982 May-Jun;2(3):129-32.
Two hundred 13 week old male and female C3H/HeJ mice were used to gauge the histological effects of a coupled tumor protein antigen (CTPA) on the development of transplanted tumors in these inbred mice. A group of the animals was transplanted with 2mm3 pieces of neoplastic tissue derived from a single spontaneous adenocarcinoma excised from a retired breeder C3H/HeJ female mouse. One hundred of these transplanted animals were subsequently injected with 0.2 ml of CTPA via a hind foot pad at weekly intervals, while others were left untreated. The transplanted tumors were allowed to develop for six weeks, at which time they were excised both from treated and nontreated animals, prepared according to standard histological techniques and stained with hematoxylin and eosin. Examination of the stained tissues revealed numerous chronic inflammatory cells in neoplasms from transplanted CTPA-treated animals. Tissue disruption, lymphocytic infiltration and macrophage-mediated phagocytosis were in evidence. In contrast, analogous tissues from transplanted control mice did not exhibit any manifestations of cellular immunity or chronic inflammation. It would appear, then, that a degree of cell-mediated immunity was induced in animals treated with CTPA.
选用200只13周龄的C3H/HeJ雌雄小鼠，以评估偶联肿瘤蛋白抗原（CTPA）对这些近交系小鼠移植瘤生长的组织学影响。一组动物移植了从一只退役繁殖种C3H/HeJ雌性小鼠切除的单个自发性腺癌的2mm³肿瘤组织块。随后，其中100只移植动物每周通过后足垫注射0.2 ml CTPA，而其他动物不做处理。让移植瘤生长6周，此时将处理组和未处理组动物的肿瘤切除，按照标准组织学技术制备，并进行苏木精和伊红染色。对染色组织的检查显示，来自接受CTPA处理的移植动物肿瘤中有大量慢性炎症细胞。可见组织破坏、淋巴细胞浸润和巨噬细胞介导的吞噬作用。相比之下，来自移植对照小鼠的类似组织未表现出任何细胞免疫或慢性炎症的表现。那么，似乎在用CTPA处理的动物中诱导了一定程度的细胞介导免疫。
