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用乳糖-蛋黄-甘油缓冲液稀释并在5℃保存的精液人工授精的全舍饲绵羊的繁殖性能。

Reproductive performance of totally confined sheep bred with semen extended in a lactose-egg yolk-glycerol buffer and stored at 5 degrees C.

作者信息

Hackett A J, Wolynetz M S

出版信息

Can J Comp Med. 1982 Jul;46(3):327-33.

Abstract

Two experiments were carried out in January and May 1975 to study reproductive performance (fertility, prolificacy and fecundity) of totally confined sheep synchronized for estrus. The main objective of the first experiment was to compare reproductive performance of ewes bred naturally to those bred by artificial insemination. Semen for artificial insemination was collected by either artificial vagina or electroejaculation and stored for up to 36 hours at 5 degrees C. A second objective was to evaluate the effect of pregnant mares' serum gonadotrophin on the reproductive performance of ewes synchronized for estrus. Fertility was 53% for ewes bred by natural mating, 34% for ewes receiving pregnant mares' serum gonadotrophin and bred by artificial insemination, and 9% for those not receiving pregnant mares' serum gonadotrophin and bred by artificial insemination. There was no difference in fertility between ewes bred with semen collected by artificial vagina or by electroejaculation. A similar pattern was observed for fecundity. Average prolificacy was 2.0 with no significant differences among mating methods or pregnant mares' serum gonadotrophin application being observed. In the second experiment, the reproductive performance of ewes inseminated with semen stores at 5 degrees C for 48 to 60 hours was compared to that of ewes inseminated with semen stores for 24 to 36 hours. No significant differences in the reproductive performance were detected. Although average fertility (9%) and fecundity (19%) were low, prolificacy averaged 2.0 lambs per ewe lambing.

摘要

1975年1月和5月进行了两项实验,以研究完全圈养且经同期发情处理的绵羊的繁殖性能(受孕率、产仔数和繁殖力)。第一个实验的主要目的是比较自然交配母羊与人工授精母羊的繁殖性能。人工授精所用精液通过人工阴道或电刺激采精获得,并在5摄氏度下保存长达36小时。第二个目的是评估孕马血清促性腺激素对经同期发情处理的母羊繁殖性能的影响。自然交配母羊的受孕率为53%,接受孕马血清促性腺激素并进行人工授精的母羊受孕率为34%,未接受孕马血清促性腺激素并进行人工授精的母羊受孕率为9%。通过人工阴道或电刺激采精获得的精液用于授精的母羊,其受孕率没有差异。繁殖力也观察到类似模式。平均产仔数为2.0,不同交配方式或是否应用孕马血清促性腺激素之间未观察到显著差异。在第二个实验中,将用在5摄氏度下保存48至60小时的精液进行授精的母羊的繁殖性能,与用保存24至36小时的精液进行授精的母羊的繁殖性能进行了比较。未检测到繁殖性能的显著差异。尽管平均受孕率(9%)和繁殖力(19%)较低,但每只产羔母羊的平均产仔数为2.0只羔羊。

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