Iwasaki Y, Minamoto N
Comp Immunol Microbiol Infect Dis. 1982;5(1-3):1-8. doi: 10.1016/0147-9571(82)90008-x.
A persistent infection of CVS strain of rabies virus was established in murine neuroblastoma cells, C-1300, by a serial passage of the cells infected with a low input multiplicity. Little cytopathic effects were seen in the infected cultures and the cell growth was not interfered, although 90-100% of the cells were bearing intracytoplasmic inclusions and the infectious virus was constantly recovered from the supernatant. Scanning electron microscopy disclosed preferential budding of the virus from fine cellular processes with a relative sparing of the cell body. Freeze-fracture of the infected cells revealed that the intracytoplasmic inclusion (matrix) was composed of an aggregate of fine particles with a diameter of approximately 200 A. Intramembrane particles were distributed sparsely and randomly in the viral envelope.
通过以低感染复数连续传代感染鼠神经母细胞瘤细胞C-1300，建立了狂犬病病毒CVS株的持续感染。尽管90%-100%的细胞含有胞质内包涵体，且上清液中持续可回收感染性病毒，但在感染培养物中几乎未见细胞病变效应，细胞生长也未受干扰。扫描电子显微镜显示病毒优先从细胞的细微突起处出芽，而细胞体相对较少受累。感染细胞的冷冻蚀刻显示，胞质内包涵体（基质）由直径约200埃的细颗粒聚集体组成。膜内颗粒在病毒包膜中稀疏且随机分布。
