Hermanský F, Poch T, Lodrová V
Histochemistry. 1977 Jul 18;53(1):89-95. doi: 10.1007/BF00511213.
A selective staining of hemoglobin in erythroid cell series was achieved by use of Sudan Black B (modified method of Sheehan and Storey) if optimal amount of hydrogen peroxide was added to the staining mixture. The effect of some inhibitory agents (KCN, wet heat, pH) on this staining as well as on the Lepehne's pseudoperoxidase reaction for hemoglobin was similar. Both reactions were more resistant to these factors than the peroxidase reactions and sudanophilia in granulocytes in which both could be blocked by the pretreatment with absolute methanol. Moreover the effect of some extraction procedures for lipids on both myeloperoxidase reactions and sudanophilia was investigated. The results support the view that the sudanophilia in granulocytes is due to their peroxidase activity and for the staining of hemoglobin by use of Sudan Black B with H2O2 its pseudoperoxidase activity is responsible. In addition the effect of the substitution of phenolphosphate by dihydroxybenzenes on granulocyte sudanophilia is reported.
如果在染色混合液中加入适量的过氧化氢，使用苏丹黑B（希恩和斯托里改良法）可实现红细胞系血红蛋白的选择性染色。一些抑制剂（氰化钾、湿热、pH值）对这种染色以及对血红蛋白的勒佩内假过氧化物酶反应的影响相似。这两种反应比过氧化物酶反应以及粒细胞中的嗜苏丹性更耐这些因素，在粒细胞中，用无水甲醇预处理可阻断这两种反应。此外，还研究了一些脂质提取程序对髓过氧化物酶反应和嗜苏丹性的影响。结果支持这样的观点，即粒细胞中的嗜苏丹性是由于其过氧化物酶活性，而用苏丹黑B和过氧化氢对血红蛋白进行染色则是由其假过氧化物酶活性所致。此外，还报道了用二羟基苯替代磷酸苯酯对粒细胞嗜苏丹性的影响。
