二丁酰环磷腺苷对热疗后L5178Y细胞蛋白质合成的影响。

Effect of dibutyryl cyclic adenosine 5'-monophosphate on protein synthesis in L5178Y cells after hyperthermia.

作者信息

Fuhr J E, Yang T J, Townes T, Overton M

出版信息

J Natl Cancer Inst. 1977 Nov;59(5):1469-73. doi: 10.1093/jnci/59.5.1469.

DOI:10.1093/jnci/59.5.1469

Abstract

Protein synthesis in the L5178Y mouse lymphoma cell line was stimulated after short-duration heat shock by the addition of dibutyryl cyclic AMP. The stimulation was concentration-dependent (range, 0.01-1.0 mM) and was enhanced by preincubation of the cells in the presence of the cyclic nucleotide before the heat shock. Actinomycin, which blocks the recovery of protein synthesis activity after heat shock, did not block the cyclic AMP stimulation.

摘要

在L5178Y小鼠淋巴瘤细胞系中，短暂热休克后添加二丁酰环磷腺苷可刺激蛋白质合成。这种刺激呈浓度依赖性（范围为0.01 - 1.0 mM），并且在热休克前将细胞在环核苷酸存在下预孵育可增强这种刺激。放线菌素可阻断热休克后蛋白质合成活性的恢复，但并不阻断环磷腺苷的刺激作用。