2',5'-腺苷酸对促红细胞生成素依赖性集落形成的抑制作用
2'5'-adenylate inhibition of erythropoietin-dependent colony formation.
作者信息
Orlic D, Quaini F, Konski A A, Wu J M
出版信息
Stem Cells (1981). 1982;1(4-5):261-8.
The tetramer core of 2'5'-adenylate was tested in vitro for its effect on erythroid colony-forming units (CFU-E) from bone marrow of adult rats. Inhibition of CFU-E was 2'5'-adenylate dose dependent, when tested with concentrations of 0.05- 1.0 mM. Also, 2'5'-adenylate was inhibitory when incubated with 2, 6 or 10 X 10(4) cells/0.1 ml plasma clot. At 0.1 mM concentration of 2'5'-adenylate there was a 30-50% reduction of erythroid colonies for each of these three cell concentrations. There was an early decrease in 3H-thymidine incorporation when 2'5'-adenylate was added to erythropoietin-stimulated bone marrow cell suspensions prior to plating. It is suggested that in situ production of 2'5'-adenylate may represent a regulatory mechanisms in red cell maturation.
对2',5'-腺苷酸的四聚体核心进行了体外测试，以研究其对成年大鼠骨髓中红系集落形成单位（CFU-E）的影响。当使用0.05 - 1.0 mM的浓度进行测试时，CFU-E的抑制作用呈2',5'-腺苷酸剂量依赖性。此外，当与2、6或10×10⁴个细胞/0.1 ml血浆凝块一起孵育时，2',5'-腺苷酸具有抑制作用。在2',5'-腺苷酸浓度为0.1 mM时，对于这三种细胞浓度中的每一种，红系集落减少30 - 50%。在接种前将2',5'-腺苷酸添加到促红细胞生成素刺激的骨髓细胞悬液中时，³H-胸腺嘧啶核苷掺入量早期就会减少。有人提出，2',5'-腺苷酸的原位产生可能代表红细胞成熟过程中的一种调节机制。
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