Müller H W, Seifert W
J Neurosci Res. 1982;8(2-3):195-204. doi: 10.1002/jnr.490080209.
A nearly pure neuronal culture from embryonic rat brain (hippocampus) has been established in order to observe individual neurons during their in vitro development. This neuronal culture has been used as a test system for a neurotrophic factor (NTF) which is released into a serum-free defined medium, presumably by astrocytes, the dominant cell type in a primary glial culture from rat brain. NTF is essential for the development of the hippocampal neurons in cell culture. The number of neurons responding to NTF by neurite extension is highly dependent on the concentration of the factor in the culture medium. The amount of factor in serum-free glial conditioned medium can be estimated in a rapid (20-hour) bioassay.
为了在体外培养过程中观察单个神经元，已建立了一种来自胚胎大鼠脑（海马体）的近乎纯的神经元培养物。这种神经元培养物已被用作一种神经营养因子（NTF）的测试系统，该因子被释放到无血清的限定培养基中，推测是由星形胶质细胞释放的，星形胶质细胞是大鼠脑原代胶质细胞培养中的主要细胞类型。NTF对于细胞培养中海马神经元的发育至关重要。通过神经突延伸对NTF作出反应的神经元数量高度依赖于培养基中该因子的浓度。无血清胶质条件培养基中因子的量可以通过快速（20小时）生物测定法进行估计。
