Courtens J L
Reprod Nutr Dev (1980). 1982;22(5):825-40.
The depolymerization of the microtubules of the spermatid manchette was effective for 4 to 5 h in rat, starting 30 min after one intratesticular injection of colcemid in vivo. The manchette built up again later was normal in old elongating spermatids but very oddly shaped in young elongating spermatids in which the nuclear shape was abnormal and the relations between the nucleus and the endoplasmic reticulum aberrant, ie destruction of the apparatus for nucleocytoplasmic exchange. From 24 to 96 h after the injection, a progressive shifting of the cellular associations indicated that young spermatids elongating at the time of injection did not differentiate further. This could result from the lack of two roles not played by the new manchette: the guiding effect of the intranuclear chromatin and the role of skeleton for the cytoplasmic apparatus devoted to nucleocytoplasmic exchange. Step 14 to 16 spermatids were not modified by the removal of the manchette due to correct reformation of a typical manchette 4 to 5 h later. The associations of the microtubules and the nuclei and the speculative roles of the microtubules during spermiogenesis have been discussed.
在大鼠体内，在睾丸内注射秋水仙酰胺30分钟后，精子细胞环层微管的解聚在4至5小时内有效。后来重新形成的环层在老龄伸长精子细胞中是正常的，但在幼龄伸长精子细胞中形状非常奇特，其中核形状异常，核与内质网的关系异常，即核质交换装置遭到破坏。注射后24至96小时，细胞组合的逐渐移位表明，注射时正在伸长的幼龄精子细胞没有进一步分化。这可能是由于新形成的环层没有发挥的两种作用缺失所致：核内染色质的引导作用以及作为致力于核质交换的细胞质装置骨架的作用。由于4至5小时后典型环层的正确重新形成，第14至16步精子细胞不会因环层的去除而发生改变。本文讨论了微管与细胞核的组合以及微管在精子发生过程中的推测作用。
