采用高效液相色谱法测定化妆品和药品中尿囊素及氯羟铝尿囊素盐的含量。

Determination of allantoin and chlorohydroxyaluminium allantoinate in cosmetic and pharmaceutical products by high-performance liquid chromatography.

作者信息

Kawase J, Ueno H, Tsuji K

出版信息

J Chromatogr. 1982 Dec 31;253(2):237-42. doi: 10.1016/s0021-9673(01)88381-3.

Abstract

A high-performance liquid chromatographic method is described for convenient and direct determination of allantoin derivatives, by which as little as 0.1 micrograms of the compounds can be determined without any conventional sample preparation required to eliminate interferences. Chromatographic separation on a resin-based strong cation exchanger (H+) using water as eluent is combined with sensitive and selective post-column reaction of the allantoin derivatives. After dissolution of the sample in hot water, the determinations take 12 min per sample. The relative standard deviation is less than 1.8% and quantitative recoveries are obtained. Several commercial cosmetic and pharmaceutical products were analysed successfully. The method can be applied to test the stability of the compounds in formulations.

摘要

本文描述了一种高效液相色谱法，用于方便、直接地测定尿囊素衍生物。该方法无需任何传统的消除干扰的样品前处理，就能测定低至0.1微克的这些化合物。使用水作为洗脱剂，在基于树脂的强阳离子交换剂（H+）上进行色谱分离，并结合尿囊素衍生物灵敏且具选择性的柱后反应。样品在热水中溶解后，每个样品的测定耗时12分钟。相对标准偏差小于1.8%，且获得了定量回收率。成功分析了几种商业化妆品和药品。该方法可用于测试化合物在制剂中的稳定性。