[1-¹⁴C]乙酸盐掺入杜氏利什曼原虫前鞭毛体脂肪酸和甘油脂质的脂肪族部分。

Incorporation of [1-14C]acetate into fatty acids and aliphatic moieties of glycerolipids in Leishmania donov ani promastigotes.

Jacobs G, Herrmann H, Gercken G

Comp Biochem Physiol B. 1982;73(2):367-73. doi: 10.1016/0305-0491(82)90299-1.

Abstract

Short-time incubations (20-300 sec) of Leishmania donovani with [1-14C] acetate showed lauric, myristic and palmitic acid to be labeled first, whereas from 150 sec on the radioactivity was mainly found in stearic and oleic acid. 2. After an incubation period of 7 days the distribution of radioactivity in fatty acids was similar to the mass distribution of fatty acids. 3. Up to 300 sec 14C-activity was present mainly in acyl moieties of lipids (94%). 4. Ether moieties of radylglycerols, diradylglycerophosphoethanolamine and -inositol slowly incorporated radioactivity in pulse-chase experiments from 1 to 24 hr. 5. Pulse-chase experiments revealed significant differences in plasmanylethanolamine, acylglycerol and sterol metabolism of cells in the logarithmic or stationary phase of growth.

摘要

用[1-¹⁴C]乙酸盐对杜氏利什曼原虫进行短时间孵育（20 - 300秒），结果显示月桂酸、肉豆蔻酸和棕榈酸首先被标记，而从150秒起，放射性主要出现在硬脂酸和油酸中。2. 孵育7天后，脂肪酸中放射性的分布与脂肪酸的质量分布相似。3. 长达300秒时，¹⁴C活性主要存在于脂质的酰基部分（94%）。4. 在1至24小时的脉冲追踪实验中，放射性甘油基甘油、二放射性甘油磷酸乙醇胺和 - 肌醇的醚部分缓慢掺入放射性。5. 脉冲追踪实验揭示了处于对数生长期或稳定期的细胞在血浆乙醇胺、酰基甘油和甾醇代谢方面存在显著差异。