Popov G, Martinov S
Vet Med Nauki. 1982;19(4):3-12.
A comprehensive study was carried out on the electron microscope diagnostics of Chlamydia in diseased animals, birds, and humans. Studied were ultra thin cross sections, and negative-contrast microscopy was employed in the investigation of a total of 953 clinical, pathologic, and laboratory-and-experimental samples from animals, birds, and humans suspective of being carriers of Chlamydial infections. The investigations were performed in 21 nosologic units. In more than one half of the samples there were elementary and initial bodies. It was found that the direct electron microscopy of Chlamydia in clinical and pathological materials was a very valuable method. Its use for the early morphologic indication and identification of the Chlamydial isolates in chick embryons proved highly effective. Such wider study on the diagnostic value of electron microscopy with Chlamydial infections was carried out for the first time. The results obtained spoke of the fact that it was highly efficient and promising with Chlamydia. Its basic qualities in such case were shown to consist in its promptness, demonstrativeness, and hermlessness. Besides, it proved to be much less time-consuming, up-to-date, and of relatively low primary cost. These characters of the method pointed to its superiority to the conventional morphologic methods making use of the light microscope.