利用高压液相色谱法分析大鼠背侧前列腺中的雌莫司汀结合蛋白（EBP）。

Analysis of Estramustine binding protein (EBP) in rat dorsal prostate by means of high pressure liquid chromatography.

作者信息

Yamanaka H, Yuasa H, Kosaku N, Bashirelahi N, Shida K

出版信息

Endocrinol Jpn. 1982 Dec;29(6):669-74. doi: 10.1507/endocrj1954.29.669.

DOI:10.1507/endocrj1954.29.669

Abstract

High pressure liquid chromatography (HPLC, Toyo Soda TSK-GEL G3000 SW column) was used to analyse the properties of Estramustine binding protein (EBP) in the cytosol of rat dorsal prostate. There exist in the cytosol of rat dorsal prostate two binding components having a high affinity for Estramastine. When estimated by HPLC, the molecular weights of these Estramustine binding components are 45,000-50,000 and 25,000-30,000 daltons, respectively. The binding of 3H-Estramustine to a macromolecule with a molecular weight of 25,000-30,000 is more heat labile than binding of 3H-Estramustine to a macromolecule with a molecular weight of 45,000-50,000. The present study also demonstrates that the HPLC method offers higher resolution, smaller sample size and faster analysis than other methods used in binding studies.

摘要

采用高压液相色谱法（HPLC，东洋曹达TSK-GEL G3000 SW柱）分析大鼠背侧前列腺胞质溶胶中雌莫司汀结合蛋白（EBP）的特性。大鼠背侧前列腺胞质溶胶中存在两种对雌莫司汀具有高亲和力的结合成分。通过HPLC估算，这些雌莫司汀结合成分的分子量分别为45,000 - 50,000道尔顿和25,000 - 30,000道尔顿。与分子量为45,000 - 50,000的大分子相比，3H-雌莫司汀与分子量为25,000 - 30,000的大分子的结合对热更不稳定。本研究还表明，与结合研究中使用的其他方法相比，HPLC方法具有更高的分辨率、更小的样本量和更快的分析速度。