Moody-Corbett F, Cohen M W
J Embryol Exp Morphol. 1982 Dec;72:53-69.
In culture, myotomal muscle cells from Xenopus laevis embryos develop discrete patches of high acetylcholine receptor (AChR) density. To examine the relative adhesiveness of these sites, muscle cells having AChR patches on their lower surface (apposed to the culture dish) were identified and were then treated with dibucaine or potassium-Ringer in order to cause the cells to round up. More than 90% of these cells remained attached at an AChR patch after rounding up, and this was the case even when the cells had a single patch on their lower surface. When the cells were torn away from the culture dish by mechanical agitation, small cellular fragments still remained firmly attached to the dish and many of these fragments contained an AChR patch. It is concluded that AChR patches on the lower surface of the cell are often located at sites of increased adhesiveness to the culture dish. The findings are discussed in terms of the formation and maintenance of AChR patches.
在培养过程中，非洲爪蟾胚胎的肌节肌细胞会形成乙酰胆碱受体（AChR）密度较高的离散斑块。为了检测这些位点的相对黏附性，鉴定出下表面（与培养皿相对）有AChR斑块的肌细胞，然后用丁卡因或钾-林格液处理以使细胞变圆。超过90%的这些细胞变圆后仍通过一个AChR斑块附着，即便细胞下表面只有一个斑块时也是如此。当通过机械搅拌将细胞从培养皿上撕脱时，小的细胞碎片仍牢固地附着在培养皿上，并且许多这些碎片含有一个AChR斑块。得出的结论是，细胞下表面的AChR斑块常位于与培养皿黏附性增加的位点。根据AChR斑块的形成和维持对这些发现进行了讨论。
