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与脱氧镰状血红蛋白纤维相关的三斜晶体。

Triclinic crystals associated with fibers of deoxygenated sickle hemoglobin.

作者信息

Magdoff-Fairchild B, Rosen L S, Chiu C C

出版信息

EMBO J. 1982;1(1):121-6. doi: 10.1002/j.1460-2075.1982.tb01134.x.

DOI:10.1002/j.1460-2075.1982.tb01134.x
PMID:7188178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553005/
Abstract

Triclinic crystals have been found in capillaries that initially contained deoxygenated sickled erythrocytes, and in solutions of sickle hemoglobin that were stirred during deoxygenation. In both cases these crystals occur as a phase transition from fibers. They have been observed only as twins; the a-axis of one member is related to that of its twin by 180 degrees rotation about the b* direction. The volume of the triclinic crystal unit cell is half that of the monoclinic crystals that have also transformed from fibers. Analysis of X-ray diffraction data indicates that the two molecules in the triclinic unit cell that repeat at an interval of 64 A form double filaments similar to those found in the monoclinic crystals and in the fiber. The existence of the triclinic crystals which contain only one double filament per unit cell removes a postulated requirement that antipolar double filament pairs be the sole unit of the fiber architecture.

摘要

在最初含有脱氧镰状红细胞的毛细血管中以及在脱氧过程中搅拌的镰状血红蛋白溶液中发现了三斜晶体。在这两种情况下,这些晶体都是从纤维发生相变形成的。它们仅以孪晶形式被观察到;一个成员的a轴与其孪晶的a轴通过绕b*方向180度旋转相关。三斜晶体晶胞的体积是同样由纤维转变而来的单斜晶体晶胞体积的一半。X射线衍射数据分析表明,三斜晶胞中以64 Å间隔重复的两个分子形成了类似于在单斜晶体和纤维中发现的双丝。每个晶胞仅包含一根双丝的三斜晶体的存在消除了一个假设要求,即反极性双丝对是纤维结构的唯一单元。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/eba163ebef93/emboj00293-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/bfe87fad1906/emboj00293-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/fde396c1cc53/emboj00293-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/c7e26906f3a2/emboj00293-0127-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/eba163ebef93/emboj00293-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/bfe87fad1906/emboj00293-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/fde396c1cc53/emboj00293-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/c7e26906f3a2/emboj00293-0127-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aba/553005/eba163ebef93/emboj00293-0128-a.jpg

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Triclinic crystals associated with fibers of deoxygenated sickle hemoglobin.与脱氧镰状血红蛋白纤维相关的三斜晶体。
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引用本文的文献

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Biophys J. 1986 Jan;49(1):67-9. doi: 10.1016/S0006-3495(86)83595-0.
2
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3
Computer models of a new deoxy-sickle cell hemoglobin fiber based on x-ray diffraction data.基于X射线衍射数据的新型脱氧镰状细胞血红蛋白纤维的计算机模型。

本文引用的文献

1
Double filaments in fibers and crystals of deoxygenated hemoglobin s.去氧血红蛋白S的纤维和晶体中的双丝。
Biophys J. 1980 Oct;32(1):436-8. doi: 10.1016/S0006-3495(80)84972-1.
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Deoxygenated sickle hemoglobin: phase transformation from fiber to a new monoclinic crystalline form.脱氧镰状血红蛋白:从纤维到新单斜晶型的相变。
J Mol Biol. 1980 Feb 5;136(4):455-9. doi: 10.1016/0022-2836(80)90401-5.
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Polymorphic assemblies of double strands of sickle cell hemoglobin. Manifold pathways of deoxyhemoglobin S crystallization.镰状细胞血红蛋白双链的多态性组装。脱氧血红蛋白S结晶的多种途径。
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Electron microscope study of the kinetics of the fiber-to-crystal transition of sickle cell hemoglobin.镰状细胞血红蛋白纤维到晶体转变动力学的电子显微镜研究。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):944-8. doi: 10.1073/pnas.77.2.944.
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