一种新型高效肝素的特性研究。第五篇通讯：通过抗凝血酶-琼脂糖亲和层析进一步表征及其与市售肝素的比较。
Characterization of a new potent heparin. 5th Communication: Further characterization by affinity chromatography on antithrombin-sepharose and its comparison with a commercially available heparin.
作者信息
Bhargava A S, Freihube G, Günzel P
出版信息
Arzneimittelforschung. 1980;30(10):1621-3.
A highly potent heparin (Heparin Schering) was characterized in comparison to commercial heparin by affinity chromatography on antithrombin-III-sepharose. Heparin Schering consists of 83% high affinity and 17% low affinity fractions, whereas commercial heparin contained only 40% high affinity heparin and 60% low affinity heparin. The specific biological activities per microgram uronic acid and elution pattern on antithrombin-sepharose of high affinity heparin fraction were similar for both heparin preparations. It is concluded that the 2 times more potent anticoagulant activity of heparin Schering is mainly due to the enrichment of high affinity fraction (83%), which is also present in commercial heparin but at a remarkably lower percentage (40%).
通过在抗凝血酶III-琼脂糖上进行亲和色谱分析，将一种高效力肝素（先灵葆雅肝素）与市售肝素进行了特性对比。先灵葆雅肝素由83%的高亲和力组分和17%的低亲和力组分组成，而市售肝素仅含有40%的高亲和力肝素和60%的低亲和力肝素。两种肝素制剂的高亲和力肝素组分每微克糖醛酸的比生物活性以及在抗凝血酶-琼脂糖上的洗脱模式相似。得出的结论是，先灵葆雅肝素的抗凝活性强2倍主要是由于高亲和力组分（83%）的富集，高亲和力组分在市售肝素中也存在，但比例显著较低（40%）。
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