Crichton R R, Roman F, Roland F
J Inorg Biochem. 1980 Dec;13(4):305-16. doi: 10.1016/s0162-0134(00)80251-2.
The release of iron from horse spleen ferritin by the chelating agents desferrioxamine B, rhodotorulic acid, 2,3-dihydroxybenzoate, 2,2'-bipyridyl and pyridine-2-aldehyde-2-pyridyl hydrazone (Paphy) has been studied in vitro. Ferritin prepared by classical procedures involving thermal denaturation releases its iron less effectively than ferritin isolated by a modified procedure that avoids this step. Desferrioxamine B and rhodotorulic acid are the most effective in releasing iron from both preparations of ferritin. When FMN is added, iron release by desferrioxamine B, rhodotorulic acid, and 2,3-dihydroxybenzoate was effectively blocked, whereas both bipyridyl and Paphy showed a marked simulation. A substantial increase in iron release was also observed for bipyridyl and Paphy with ascorbate; a less important increase was noted for rhodotorulic acid. EDTA exerted a marked inhibition of iron release from ferritin with rhodotorulic acid, 2,3-dihydroxybenzoate, bipyridyl, and Paphy. The effects of citrate and oxalate on iron release by the chelators was small. The effect of the concentration of flavin on iron release from ferritin by bipyridyl and desferrioxamine B have been studied. Desferrioxamine is unable to mobilize FeII from ferritin following reduction by reduced FMN, whereas bipyridyl can rapidly complex the ferrous iron. The results are discussed in the context of our current concepts of storage iron mobilization in the treatment of iron overload.
已在体外研究了螯合剂去铁胺B、红酵母氨酸、2,3-二羟基苯甲酸盐、2,2'-联吡啶和吡啶-2-醛-2-吡啶腙（Paphy）从马脾铁蛋白中释放铁的情况。通过涉及热变性的经典程序制备的铁蛋白释放铁的效率低于通过避免这一步骤的改良程序分离的铁蛋白。去铁胺B和红酵母氨酸在从两种铁蛋白制剂中释放铁方面最有效。添加FMN时，去铁胺B、红酵母氨酸和2,3-二羟基苯甲酸盐引起的铁释放被有效阻断，而联吡啶和Paphy则表现出明显的促进作用。联吡啶和Paphy与抗坏血酸盐一起时，铁释放也显著增加；红酵母氨酸的增加不太明显。EDTA对红酵母氨酸、2,3-二羟基苯甲酸盐、联吡啶和Paphy引起的铁蛋白铁释放有明显抑制作用。柠檬酸盐和草酸盐对螯合剂铁释放的影响较小。研究了黄素浓度对联吡啶和去铁胺B从铁蛋白中释放铁的影响。去铁胺在被还原型FMN还原后无法从铁蛋白中动员亚铁，而联吡啶可以迅速络合亚铁。在我们目前关于治疗铁过载中储存铁动员概念的背景下讨论了这些结果。
