Fuge H
Eur J Cell Biol. 1981 Aug;25(1):90-4.
Tannic acid as a mordant in electron microscopy has recently been successfully employed in demonstrating a prominent spindle microfilament system in an alga [21]. The same technique was applied in this study to meiotic spindles of the crane fly Pales ferruginea. Although the contrast of the intertubular spindle matrix is very much improved with this technique, no filamentous structures suggesting the existence of a microfilament system in prometa-, meta-, and anaphase spindles could be detected. This may possibly indicate that spindles of different eukaryotes exhibit profound differences in their basic composition.
单宁酸作为电子显微镜中的媒染剂，最近已成功用于显示一种藻类中突出的纺锤体微丝系统[21]。本研究将相同技术应用于大蚊的减数分裂纺锤体。尽管用该技术可大大改善纺锤体微管间基质的对比度，但在前期、中期和后期纺锤体中未检测到表明存在微丝系统的丝状结构。这可能表明不同真核生物的纺锤体在其基本组成上存在显著差异。
