Johnson L A, Aalbers J G, Willems C M, Rademaker J H, Rexroad C E
J Anim Sci. 1982 Jan;54(1):132-6. doi: 10.2527/jas1982.541132x.
A field trial was conducted on several hundred farms in The Netherlands to compare the fertilizing capacity of boar spermatozoa stored for 1, 2, or 3 d at 18 C in either Kiev or Beltsville liquid extender (BL-1). Two storage volumes (25 and 100 ml) were used. Twelve Dutch Landrace boars, all in routine commercial artificial insemination service, provided the semen in twice-a-week collections. Over an 8-wk period in March and April, 1979, 2,849 sows and gilts were inseminated. Farrowing rates were higher (P less than .0001) with Kiev extender than with BL-1 extender (69.3 vs 60.5%) when semen was used over a 3 d period. The volume at which the semen was extended and stored had no effect on any parameter. Farrowing rate declined significantly when semen stored at 18 C for more than 2 d was used (74.5% vs 64.7% with semen stored for 1 and 3 in Kiev extender; 65.9% vs 52.7% with semen stored for 1 and 3 d in BL-1 extender). The farrowing rate, total number of pigs/litter and live pigs/litter were significantly higher for sows than for gilts inseminated with similar extended semen. On the basis of the results of this trial, Kiev extender appears to be more suitable as a diluent for liquid semen than does BL-1. AI with semen extended and stored for 3 d at 18 C can be expected to result in a lower farrowing rate than AI with semen stored for 1 or 2 d.
在荷兰的几百个农场进行了一项田间试验，以比较在18摄氏度下，猪精液在基辅或贝尔茨维尔液体稀释剂（BL-1）中储存1、2或3天的受精能力。使用了两种储存体积（25毫升和100毫升）。12头荷兰长白公猪均用于常规商业人工授精服务，每周采集两次精液。在1979年3月和4月的8周时间里，对2849头母猪和后备母猪进行了人工授精。当精液使用3天时，基辅稀释剂的产仔率高于BL-1稀释剂（69.3%对60.5%，P小于0.0001）。精液稀释和储存的体积对任何参数均无影响。当使用在18摄氏度下储存超过2天的精液时，产仔率显著下降（在基辅稀释剂中，储存1天和3天的精液产仔率分别为74.5%和64.7%；在BL-1稀释剂中，储存1天和3天的精液产仔率分别为65.9%和52.7%）。用类似稀释精液人工授精的母猪，其产仔率、每窝仔猪总数和每窝活仔猪数均显著高于后备母猪。根据该试验结果，基辅稀释剂似乎比BL-1更适合作为液体精液的稀释剂。预计在18摄氏度下将精液稀释并储存3天进行人工授精，其产仔率将低于使用储存1天或2天的精液进行人工授精。
