Anderson D W, Schaefer E J, Bronzert T J, Lindgren F T, Forte T, Starzl T E, Niblack G D, Zech L A, Brewer H B
J Clin Invest. 1981 Mar;67(3):857-66. doi: 10.1172/jci110103.
The daily transport of human plasma apolipoproteins A-I and A-II, triglyceride, and total cholesterol from the thoracic duct lymph into plasma was measured in two subjects before and three subjects after renal transplantation. Lymph triglyceride transport was approximately 83% of the daily ingested fat loads, whereas lymph cholesterol transport was consistently greater than the amount of daily ingested cholesterol. Lymph apolipoprotein transport significantly (P < 0.05) exceeded the predicted apolipoprotein synthesis rate by an average of 659+/-578 mg/d for apolipoprotein A-I and 109+/-59 mg/d for apolipoprotein A-II among the five subjects. It is estimated that 22-77% (apolipoprotein A-I) and 28-82% (apolipoprotein A-II) of daily total body apolipoprotein synthesis takes place in the intestine. Lymph high density lipoprotein particles are mostly high density lipoprotein(2b) and high density lipoprotein(2a) and have a greater overall relative triglyceride content and a smaller relative cholesteryl ester content when compared with homologous plasma high density lipoproteins. The major quantity of both lymph apolipoprotein A-I (81+/-8%) and apolipoprotein A-II (90+/-11%) was found within high density lipoproteins with almost all of the remainder found in chylomicrons and very low density lipoproteins. The combined results are consistent with a major contribution of the intestine to total body synthesis of apolipoprotein A-I and apolipoprotein A-II. An important role of lymph in returning filtered apolipoprotein to plasma in association with high density lipoproteins is proposed. Accompanying the return of filtered apolipoprotein to the plasma is a probable transformation, both in size and composition, of at least some of the lymph high density lipoprotein(2b) and high density lipoprotein(2a) particles into high density lipoprotein(3).
在两名肾移植前的受试者和三名肾移植后的受试者中，测量了人血浆载脂蛋白A-I和A-II、甘油三酯以及总胆固醇每日从胸导管淋巴转运至血浆的情况。淋巴甘油三酯转运量约为每日摄入脂肪量的83%，而淋巴胆固醇转运量始终大于每日摄入胆固醇的量。在这五名受试者中，淋巴载脂蛋白转运量显著超过预测的载脂蛋白合成速率（P<0.05），载脂蛋白A-I平均超出659±578mg/d，载脂蛋白A-II平均超出109±59mg/d。据估计，每日全身载脂蛋白合成量的22%-77%（载脂蛋白A-I）和28%-82%（载脂蛋白A-II）在肠道内进行。与同源血浆高密度脂蛋白相比，淋巴高密度脂蛋白颗粒大多为高密度脂蛋白(2b)和高密度脂蛋白(2a)，其总体相对甘油三酯含量更高，相对胆固醇酯含量更低。在高密度脂蛋白中发现了大部分的淋巴载脂蛋白A-I（81±8%）和载脂蛋白A-II（90±11%），几乎所有其余部分则存在于乳糜微粒和极低密度脂蛋白中。综合结果表明，肠道对载脂蛋白A-I和载脂蛋白A-II的全身合成起主要作用。提出了淋巴在将滤过的载脂蛋白与高密度脂蛋白结合返回血浆中的重要作用。随着滤过的载脂蛋白返回血浆，至少一些淋巴高密度脂蛋白(2b)和高密度脂蛋白(2a)颗粒在大小和组成上可能会转化为高密度脂蛋白(3)。
