体液中痕量六氯环戊二烯和八氯环戊烯的测定。

Determination of trace levels of hexachlorocyclopentadiene and octachlorocyclopentene in body fluids.

作者信息

DeLeon I R, Brown N J, Cocchiara J P, Miles S K, Laseter J L, Kremer E H, Makk L

出版信息

J Anal Toxicol. 1980 Nov-Dec;4(6):314-7. doi: 10.1093/jat/4.6.314.

DOI:10.1093/jat/4.6.314

PMID:7206662

Abstract

A simple, rapid and sensitive method for the analysis of hexachlorocyclopentadiene (C5-Cl6) and octachlorocyclopentene (C5-Cl8) in blood and urine is described. The procedure requires 5.0 mL of blood or urine, and involves the isolation of the compounds from the sample by liquid-liquid extraction, followed by screening and quantification via glass-capillary column gas chromatography with electron-capture detection and confirmation by gas chromatography-mass spectrometry. The assays are suitable for the detection and identification of nanogram quantities of these compounds in body fluids with a lower detection limit of 50 ng/mL for blood and 10 ng/mL for urine.

摘要

描述了一种用于分析血液和尿液中六氯环戊二烯（C5-Cl6）和八氯环戊烯（C5-Cl8）的简单、快速且灵敏的方法。该程序需要5.0毫升血液或尿液，包括通过液-液萃取从样品中分离化合物，然后通过带有电子捕获检测的玻璃毛细管柱气相色谱进行筛选和定量，并通过气相色谱-质谱进行确认。这些测定方法适用于检测和鉴定体液中纳克量的这些化合物，血液的检测下限为50纳克/毫升，尿液的检测下限为10纳克/毫升。