Lukomska B, Olszewski W L, Engeset A
Lymphology. 1980 Dec;13(4):186-91.
Cell populations in afferent lymph of human leg were defined by surface characteristics and cytotoxic activity in 7 normal men and 9 patients with localized cancer. A higher percentage of E-rosette forming cells was found in lymph (78.5 and 83.0) than in blood (60.0 and 63.0 p less than 0.05). The percentages of lymph EA-RFC were 10.3 and 18.0, of EAC-RFC 13.1 and 8.0, of surface immunoglobulin carrying cells 3.0 and 3.1. In blood 20.6 and 18.0 percent of cells formed EA-rosettes, 23.0 and 15.6 EAC-rosettes, 5 and 9.5 contained surface immunoglobulins. The differences between lymph and blood EA- and EAC-RFC in normals were statistically significant (p less than 0.05). In cancer patients only lymph-blood differences for S Ig+ were significant (p less than 0.05). No significant differences were found between normals and cancer patients. In both groups, the natural cytotoxicity against K 562 cells was 6 times lower in lymph as compared to blood (p less than 0.05), the cytotoxicity in those with cancer was higher than in normals (p less than 0.05). The study indicates that B cells have a limited tendency toward leaving the blood circulation and migrating through tissues. Moreover natural killer cells do not seem to belong to the recirculating pool of lymphocytes.
通过表面特征和细胞毒性活性对7名正常男性和9名局部癌症患者腿部传入淋巴中的细胞群体进行了定义。发现淋巴中E花环形成细胞的百分比（78.5和83.0）高于血液中（60.0和63.0，p<0.05）。淋巴中EA-RFC的百分比分别为10.3和18.0，EAC-RFC为13.1和8.0，携带表面免疫球蛋白的细胞为3.0和3.1。血液中20.6%和18.0%的细胞形成EA花环，23.0%和15.6%形成EAC花环，5%和9.5%含有表面免疫球蛋白。正常情况下，淋巴和血液中EA-和EAC-RFC的差异具有统计学意义（p<0.05）。在癌症患者中，只有淋巴与血液中S Ig+的差异具有显著性（p<0.05）。正常人和癌症患者之间未发现显著差异。在两组中，针对K 562细胞的自然细胞毒性在淋巴中比在血液中低6倍（p<0.05），癌症患者的细胞毒性高于正常人（p<0.05）。该研究表明，B细胞离开血液循环并通过组织迁移的倾向有限。此外，自然杀伤细胞似乎不属于淋巴细胞的再循环池。
