Metabolic response to starvation. I. Relative effects of pregnancy and sex steroid administration in the rat.

Increased sensitivity to a fast with rapid onset of ketosis, hypoalaninemia, and hypoglycemia is characteristic of rat and human gestation. The present study examines the role of sex steroids in the development of these metabolic changes. Subgroups of female rate received 10 microgram of estradiol (E), 10 mg of progesterone (P), or the two in combination (E + P) s.c. daily for 5 days. Control rats (C) received sesame oil alone. Different groups were starved 12, 24 and 48 hr during days 4 and 5 or treatment. Plasma substrate and insulin responses were measured and compared to results of similar studies performed on rats in late gestation. Administration of E or P along had no significant effects on measured parameters as compared to control values. However, the combined E + P regimen resulted in significantly higher ketonemia, greater hypoalaninemia, and relatively higher triglyceride concentrations than in control groups during fasting. Plasma triglyceride, free fatty acid, and ketone concentrations in the pregnant group exceeded values of both the E + P and control animals. However, plasma free fatty acids in the E + P group were significantly lower as compared to control values during starvation. These data suggest that the combined effects of estradiol and progesterone enhance ketogenesis and hypoalaninemia independent of the fetal-placental unit during starvation in pregnancy. Since the ketogenic effect was associated with a relative suppression of plasma free fatty acids, this may be indicative of a direct action of these hormones on hepatic disposition of free fatty acids and/or the biosynthetic pathways for ketone body production.