从兔肝多核糖体mRNA模板体外合成的细胞色素P-450-LM2的鉴定。

The identification of cytochrome P-450-LM2 synthesized in vitro from a rabbit liver polysomal mRNA template.

作者信息

DiLella A G, Chiang J Y, Hutterer F, Steggles A W

出版信息

Biochim Biophys Acta. 1981 Jan 29;652(1):223-7. doi: 10.1016/0005-2787(81)90225-2.

Abstract

We demonstrate the in vitro synthesis of cytochrome P-450-LM2 (phenobarbital-inducible form of liver microsomal cytochrome P-450) from a rabbit liver polysomal mRNA template by specific immunoprecipitation of the product. The in vitro synthesized cytochrome P-450-LM2 comigrates with authentic cytochrome P-450-LM2 on sodium dodecyl sulphate-polyacrylamide gels, and is also selectively competed by authentic purified cytochrome P-450-LM2. In addition, we demonstrate that phenobarbital increases the amount of translatable mRNA for cytochrome P-450-LM2 but not for albumin, suggesting that phenobarbital has selective effects on the amount of available translatable mRNA or on mRNA biosynthesis.

摘要

我们通过对产物进行特异性免疫沉淀，证明了从兔肝多聚核糖体mRNA模板体外合成细胞色素P-450-LM2（苯巴比妥诱导的肝微粒体细胞色素P-450形式）。体外合成的细胞色素P-450-LM2在十二烷基硫酸钠-聚丙烯酰胺凝胶上与天然细胞色素P-450-LM2共迁移，并且也被天然纯化的细胞色素P-450-LM2选择性竞争。此外，我们证明苯巴比妥增加了细胞色素P-450-LM2而非白蛋白的可翻译mRNA量，这表明苯巴比妥对可用可翻译mRNA量或mRNA生物合成具有选择性作用。