Packard D S
J Embryol Exp Morphol. 1980 Oct;59:113-30.
A simple, reliable method for the in vitro cultivation of snapping turtle embryos was demonstrated. This technique was used to study somite formation in explants containing segmental plates. Segmental plates formed a full complement of somites whether the neural tube or the neural tube and notochord was present. Explanted snapping turtle segmental plates formed an average of 6.6 +/- 1.2 somites. Removal of the node region or tail bud from cultured intact embryos led to a cessation of somite formation after an additional 6.1 +/- 1.8 somites had formed. These results indicate the number of somites the snapping turtle segmental plate will form. Also, the number of somites formed by explanted segmental plates showed only slight variation over a wide range of segmental plate lengths. It was concluded that while snapping turtle segmental plates formed significantly fewer somites than chicken or Japanese quail segmental plates, they were similar to the avian explants in their ability to form a consistent number of somites regardless of the length of the segmental plate.
展示了一种用于体外培养鳄龟胚胎的简单、可靠方法。该技术用于研究含有体节板的外植体中的体节形成。无论神经管或神经管和脊索是否存在，体节板都会形成完整的体节。移植的鳄龟体节板平均形成6.6±1.2个体节。从培养的完整胚胎中去除节点区域或尾芽，在额外形成6.1±1.8个体节后，体节形成停止。这些结果表明鳄龟体节板将形成的体节数量。此外，移植的体节板形成的体节数量在很宽的体节板长度范围内仅显示出轻微变化。得出的结论是，虽然鳄龟体节板形成的体节明显少于鸡或日本鹌鹑的体节板，但它们在形成一致数量体节的能力方面与鸟类外植体相似，而与体节板的长度无关。
