25-羟基-24-氧代维生素D3的分离、鉴定及生物活性：维生素D3经肾匀浆体外孵育产生的一种新代谢产物

Isolation, identification, and biological activity of 25-hydroxy-24-oxovitamin D3: a new metabolite of vitamin D3 generated by in vitro incubations with kidney homogenates.

作者信息

Takasaki Y, Suda T, Yamada S, Takayama H, Nishii Y

出版信息

Biochemistry. 1981 Mar 17;20(6):1681-6. doi: 10.1021/bi00509a042.

Abstract

A metabolite of 25-hydroxyvitamin D3 has been isolated in pure form from incubation mixtures containing kidney homogenates of chicks given large doses of vitamin D3. The isolation involved methanol--chloroform extraction and six steps of column chromatography. The metabolite was identified as 25-hydroxy-24-oxovitamin D3 by means of ultraviolet absorption spectrometry, mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and specific chemical reactions. Use of a sensitive in situ technique revealed that 25-hydroxy-24-oxovitamin D3 enhances intestinal calcium transport in rats approximately as effectively as 24,25-dihydroxyvitamin D3 does. In contrast, 25-hydroxy-24-oxovitamin D3 appeared to be less active than 24,25-dihydroxyvitamin D3 in chicks 24 h after intravenous injection.

摘要

从给大剂量维生素D3的雏鸡肾脏匀浆的孵育混合物中，已以纯形式分离出一种25-羟基维生素D3的代谢产物。分离过程包括甲醇 - 氯仿萃取和六步柱色谱法。通过紫外吸收光谱法、质谱法、红外光谱法、核磁共振光谱法和特定化学反应，将该代谢产物鉴定为25-羟基-24-氧代维生素D3。使用一种灵敏的原位技术显示，25-羟基-24-氧代维生素D3增强大鼠肠道钙转运的效果与24,25-二羟基维生素D3大致相同。相比之下，在静脉注射后24小时的雏鸡中，25-羟基-24-氧代维生素D3的活性似乎低于24,25-二羟基维生素D3。