Denburg J A, Befus A D, Goodacre R, Bienenstock J
Exp Hematol. 1981 Mar;9(3):214-22.
Histamine has been measured by an isotopic enzyme conversion assay in guinea pig bone marrow cultures under conditions which stimulate basophilopoiesis. A high degree of correlation was observed between histamine values and basophil counts in suspension cultures. Cultures of normal marrow with splenic conditioned medium (CM) prepared from spleen cells of ovalbumin (OA)-treated animals or coculture of marrow cells from these animals with autologous spleen cells demonstrated rises in histamine values which paralleled basophil counts, with a mean calculated histamine of 0.3 pg/basophil. Addition to marrow cells from OA-treated animals of autologous splenic T-lymphocytes or culture of normal marrow in the presence of CM derived from PHA-stimulated splenic T-lymphocytes caused in vitro increases in histamine significantly greater than when T-lymphocyte depleted spleen cells or CM derived from the latter were used, respectively (P less than 0.02). The presence of OA in marrow-spleen cultures significantly enhanced basophilopoiesis when whole or T-enriched, but not T-depleted, spleen fractions were used (P less than 0.02). The magnitude of in vitro increases in histamine over one week was 10-30 nanograms, accompanied by appropriate increases in basophils in CM-stimulated normal marrow cultures. From these data it can be concluded that histamine is an independent criterion of basophilopoiesis in vitro. An entirely new population of histamine-synthesizing cells appears to arise over 1 week in vitro under conditions of antigen or T-cell product stimulation.
在刺激嗜碱性粒细胞生成的条件下，已通过同位素酶转化测定法在豚鼠骨髓培养物中测量了组胺。在悬浮培养物中，观察到组胺值与嗜碱性粒细胞计数之间存在高度相关性。用卵清蛋白（OA）处理的动物的脾细胞制备的脾条件培养基（CM）培养正常骨髓，或将这些动物的骨髓细胞与自体脾细胞共培养，结果显示组胺值升高，与嗜碱性粒细胞计数平行，计算得出的平均组胺为每嗜碱性粒细胞0.3皮克。将来自OA处理动物的自体脾T淋巴细胞添加到骨髓细胞中，或在PHA刺激的脾T淋巴细胞衍生的CM存在下培养正常骨髓，体外组胺增加量显著大于分别使用T淋巴细胞耗尽的脾细胞或后者衍生的CM时（P小于0.02）。当使用完整的或富含T细胞的脾部分（而非T细胞耗尽的脾部分）时，骨髓-脾培养物中OA的存在显著增强了嗜碱性粒细胞生成（P小于0.02）。在CM刺激的正常骨髓培养物中，一周内体外组胺增加量为10-30纳克，同时嗜碱性粒细胞相应增加。从这些数据可以得出结论，组胺是体外嗜碱性粒细胞生成的一个独立标准。在抗原或T细胞产物刺激条件下，体外培养1周后似乎会出现全新的组胺合成细胞群体。
