De Nicola A F, Tornello S, Weisenberg L, Fridman O, Birmingham M K
Horm Metab Res. 1981 Feb;13(2):103-6. doi: 10.1055/s-2007-1019185.
The uptake of [3H]-aldosterone by the brain and anterior pituitary (AP) was studied after i.v. injection of the isotope into adrenalectomized rats. The AP showed the higher uptake ratio (i.e., radioactivity in tissue/radioactivity in blood), while the brain regions examined (hippocampus, hypothalamus, amygdala, cerebellum and cortex) contained lower levels of radioactivity, although they concentrated the hormone from blood. Neither [3H]-corticosterone nor [3H]-18-hydroxydeoxycorticosterone accumulated in the AP as much as [3H]-aldosterone, while [3H]-corticosterone's uptake was greatest in the hippocampus. Competition experiments demonstrated that [3H]-aldosterone uptake in the AP was inhibited by pretreatment of animals with excess aldosterone, corticosterone and dexamethasone, whereas aldosterone and corticosterone but not dexamethasone competed in the brain regions. Binding sites were demonstrated in vitro in cytosol fractions from AP and several brain regions. Scatchard plot analysis demonstrated high affinity, low capacity binding sites in cytosol from AP and hippocampus. These results suggest the AP and brain areas may be considered as targets for aldosterone, although the functions of the mineralocorticoid in these tissues are a matter of speculation.
在给肾上腺切除的大鼠静脉注射[3H]-醛固酮后，研究了大脑和垂体前叶（AP）对其的摄取情况。垂体前叶显示出较高的摄取率（即组织中的放射性/血液中的放射性），而所检查的脑区（海马体、下丘脑、杏仁核、小脑和皮层）放射性水平较低，尽管它们能从血液中浓缩该激素。[3H]-皮质酮和[3H]-18-羟脱氧皮质酮在垂体前叶中的积累都不如[3H]-醛固酮多，而[3H]-皮质酮在海马体中的摄取量最大。竞争实验表明，用过量醛固酮、皮质酮和地塞米松预处理动物会抑制垂体前叶对[3H]-醛固酮的摄取，而醛固酮和皮质酮而非地塞米松在脑区存在竞争。在垂体前叶和几个脑区的胞质溶胶组分中进行了体外结合位点的证明。Scatchard图分析表明，垂体前叶和海马体的胞质溶胶中存在高亲和力、低容量的结合位点。这些结果表明，垂体前叶和脑区可能被视为醛固酮的作用靶点，尽管盐皮质激素在这些组织中的功能尚属推测。
