Kaneko Y
Horm Metab Res. 1981 Feb;13(2):110-4. doi: 10.1055/s-2007-1019187.
Daunomycin (DM), a potent chemotherapeutic agent, was linked to bovine thyrotropin (TSH) directly (TSH-DM) and indirectly (TSH-alpha-lactalbumin-DM) by covalent cross-linking methods. The activity of these hormone-daunomycin conjugates was tested in vitro on the rabbit thyroid cells in primary culture. Both TSH-DM and TSH-alpha-lactalbumin-DM conjugates inhibited [3H]leucine incorporation and cell proliferation, while free TSH did not. Their inhibitory action was more potent than that of free daunomycin. The inhibition appeared to be specific to the thyroid cells and diminished in the presence of a large amount of free TSH, suggesting that the cytotoxic action of these TSH-DM conjugates was a receptor-mediated phenomenon.
柔红霉素（DM）是一种强效化疗药物，通过共价交联方法直接（TSH-DM）和间接（TSH-α-乳白蛋白-DM）与牛促甲状腺激素（TSH）相连。这些激素-柔红霉素偶联物的活性在体外对原代培养的兔甲状腺细胞进行了测试。TSH-DM和TSH-α-乳白蛋白-DM偶联物均抑制[3H]亮氨酸掺入和细胞增殖，而游离TSH则无此作用。它们的抑制作用比游离柔红霉素更强。这种抑制似乎对甲状腺细胞具有特异性，并且在存在大量游离TSH的情况下减弱，这表明这些TSH-DM偶联物的细胞毒性作用是一种受体介导的现象。
