肝动脉化可改善门腔分流大鼠肝细胞的超微结构。

Liver arterialization improves hepatocytes ultrastructure in rats with portacaval shunts.

作者信息

Saric J, Faugon H, Beliard R, Perissat J, Balabaud C, Dubuisson L, Bioulac P

出版信息

Dig Dis Sci. 1981 Mar;26(3):225-31. doi: 10.1007/BF01391634.

Abstract

The effect of arterialization (ART) of the distal stump of the portal vein after portacaval shunt (PCS) on bile formation and liver ultrastructure was assessed. ART using the left gastric artery was performed in male Wistar rats. Animals were sacrificed 3 weeks later. ART prevented body and liver atrophy. However, the liver weight to body weight ratio was significantly decreased when compared to sham PCS (2.5 +/- 0.36 vs 3.22 +/- 0.15). Reduction in total bile secretion (microliter/min) seen following PCS is reversed by ART. ART partly corrected hepatocyte size atrophy and the major ultrastructural abnormalities, namely the irregularity of the nucleus and dilation of the nuclear envelope and of the rough endoplasmic reticulum appearing after PCS. However, mitochondria remained swollen, deformed and enlarged with scission figures. No lesions in connection with ART were seen. This result confirms, at the ultrastructural level, the beneficial effect of ART in PCS.

摘要

评估门腔分流术（PCS）后门静脉远端残端动脉化（ART）对胆汁形成和肝脏超微结构的影响。在雄性Wistar大鼠中使用胃左动脉进行动脉化。3周后处死动物。动脉化可防止身体和肝脏萎缩。然而，与假手术组的门腔分流术相比，肝脏重量与体重之比显著降低（2.5±0.36对3.22±0.15）。门腔分流术后总胆汁分泌量（微升/分钟）的减少可通过动脉化逆转。动脉化部分纠正了肝细胞大小萎缩以及主要的超微结构异常，即门腔分流术后出现的细胞核不规则、核膜扩张和粗面内质网扩张。然而，线粒体仍然肿胀、变形且增大，并出现分裂象。未发现与动脉化相关的病变。这一结果在超微结构水平上证实了动脉化在门腔分流术中的有益作用。