小牛卫星I DNA的1360 bp长的基本重复单元包含约140 bp的富含GC的核。

The 1360 bp long basic repeat unit of calf satellite I DNA contains GC rich nucleus of about 140 bp.

作者信息

Skowroński J, Furtak K, Kłysik J, Panusz H, Płucienniczak A

出版信息

Nucleic Acids Res. 1978 Nov;5(11):4077-85. doi: 10.1093/nar/5.11.4077.

DOI:10.1093/nar/5.11.4077

PMID:724508

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342735/

Abstract

Fine melting profiles of calf satellite I DNA and its fragments obtained after digestion with endoR.EcoRI and endoR.AluI nucleases were investigated. It is shown that the 1360 bp basic repeat unit of calf satellite I DNA contains an about 140 bp long GC rich nucleus. It is localized on the 600 bp restriction fragment obtained after digestion of 1360 bp fragment with endoR.AluI nuclease. The main part of satellite I DNA melts as loops between such GC rich nuclei which strongly influence the melting properties of this satellite. There exist significant differences between the thermal stabilities of fragments containing many nuclei, one nucleus and those in which such nucleus is absent.

摘要

研究了小牛卫星I DNA及其经内切酶R.EcoRI和内切酶R.AluI消化后获得的片段的精细解链图谱。结果表明，小牛卫星I DNA的1360 bp基本重复单元包含一个约140 bp长的富含GC的核。它位于用内切酶R.AluI消化1360 bp片段后获得的600 bp限制性片段上。卫星I DNA的主要部分以富含GC的核之间的环的形式解链，这些核强烈影响该卫星的解链特性。含有多个核、一个核以及不含此类核的片段的热稳定性存在显著差异。