Binding of Triton X-114 to mammalian serum albumins at critical micelle concentration.

When the nonionic detergent Triton X-114 binds to different mammalian serum albumins, remarkably little variation occurs in the number bound at the critical micelle concentration of the detergent. Average molar ratios range from 4.0 to 6.3; mean body weights range from 0.25 to 600 kg. Measured values of the extinction coefficients reported here also show remarkably little variation. These results support the view that the folding of these serum albumins is similar.