Casey M L, Gal D, Johnston J M, MacDonald P C
Cancer Res. 1981 Jul;41(7):2581-2.
Prostaglandins may occupy an important role in viral and chemical carcinogen-induced neoplasia. To evaluate the possible role of prostaglandin catabolism in neoplastic cells, we measured nicotinamide adenine dinucleotide-dependent 15-hydroxyprostaglandin dehydrogenase activity in hydatidiform mole tissue and in choriocarcinoma cells maintained in monolayer culture. The specific activity of nicotinamide adenine dinucleotide-dependent 15-hydroxyprostaglandin dehydrogenase in hydatidiform mole tissue (0 to 1.2 nmol 15-ketoprostaglandin E2 formed x min-1 x mg-1 cytosolic protein) and in choriocarcinoma cells (1.0 nmol 15-ketoprostaglandin E2 x min-1 x mg-1 protein) was strikingly less than that found in normal placental tissue [11.4 +/- 2.3 (S.E.) nmol 15-ketoprostaglandin x min-1 x mg-1 protein].
前列腺素可能在病毒和化学致癌物诱导的肿瘤形成中起重要作用。为了评估前列腺素分解代谢在肿瘤细胞中的可能作用，我们测定了葡萄胎组织和单层培养的绒毛膜癌细胞中烟酰胺腺嘌呤二核苷酸依赖性15-羟基前列腺素脱氢酶的活性。葡萄胎组织（每分钟每毫克胞质蛋白形成0至1.2纳摩尔15-酮基前列腺素E2）和绒毛膜癌细胞（每分钟每毫克蛋白形成1.0纳摩尔15-酮基前列腺素E2）中烟酰胺腺嘌呤二核苷酸依赖性15-羟基前列腺素脱氢酶的比活性显著低于正常胎盘组织中的比活性[每分钟每毫克蛋白形成11.4±2.3（标准误）纳摩尔15-酮基前列腺素]。
