Barckhaus R H, Krefting E R, Althoff J, Quint P, Höhling H J
Cell Tissue Res. 1981;217(3):661-6. doi: 10.1007/BF00219372.
Dry thin sections (300-500 nm thick) of shock-frozen, freeze-dried and embedded epiphyseal growth plates from the proximal tibia of guinea pigs were cut longitudinally from the plate. Dark round bodies (phi less than 0.5 micron) were observed using the scanning transmission mode of the electron microscope initially directly in the vicinity of the chondrocytes. They gradually spread out in the direction of the metaphysis to the center of the longitudinal septum and represent most probably the matrix vesicles. By use of a microscan of 0.25 X 0.25 micron the element-concentrations of these bodies were measured. The measurements started on those bodies that could be clearly recognized and were extended to a length of 30-40 micron in the metaphyseal direction. To obtain approximate quantitative results the registered CaK alpha and PK alpha x-ray counts were directly compared with counts of fully mineralized regions, the Ca and P contents of which are known. Ca as well as p could be detected in the first visible vesicle-like structures (Ca approximately 0.2%, P approximately 0.4%) and increased steeply in the metaphyseal direction, amounting to approximately 6% Ca and 3% P. These results may lead to the conclusion that Pi becomes split from phosphate esters and transformed into the matrix vesicles already in a very early stage of enrichment. Incorporation of Ca may be coupled with this process.
将豚鼠胫骨近端经冲击冷冻、冻干并包埋的骨骺生长板制成干燥薄片（300 - 500纳米厚），从生长板纵向切片。最初在软骨细胞附近直接使用电子显微镜的扫描透射模式观察到暗圆形物体（直径小于0.5微米）。它们逐渐向干骺端方向扩散至纵向隔膜中心，很可能代表基质小泡。通过使用0.25×0.25微米的微扫描测量这些物体的元素浓度。测量从那些能够清晰识别的物体开始，并在干骺端方向延伸至30 - 40微米的长度。为了获得近似定量结果，将记录的钙Kα和磷Kα x射线计数直接与完全矿化区域的计数进行比较，已知其钙和磷含量。在最初可见的囊泡状结构中可检测到钙和磷（钙约0.2%，磷约0.4%），并在干骺端方向急剧增加，钙含量约为6%，磷含量约为3%。这些结果可能得出结论，即磷酸根离子在富集的非常早期阶段就从磷酸酯中分离出来并转化为基质小泡。钙的掺入可能与这个过程相关。
