Foppoli J M, Benedict A A
Adv Exp Med Biol. 1977;88:381-90. doi: 10.1007/978-1-4613-4169-7_36.
An alloantiserum recognizing a genetic marker (CM-1.4) on chicken 17S Ig was generated by immunizing jungle fowl with Salmonella O-anti-Salmonella O (UCD 3) agglutinates. Specificities CM-1.4 and M1a were present on 7S subunits and H chains based on their ability to inhibit in RIA the binding of alloantibody (anti-CM-1.4 or anti-M1a) to UCD 3 125I-17S Ig. CM-1.4 appears to be fully expressed on 7S subunits but is altered on H chains. In contrast, anti-M1a bound both 7S subunits and H chains with decreased avidity indicating that the M1a specificity was altered in both preparations. Since CM-1.4 and M1a are present on 17S Ig H chains, and absent from 7S Ig, they probably represent allotypic markers in the constant region of 17S Ig H chains. Having assigned two 17S Ig allotypic specificities to H chains, we propose that the locus controlling their expression be called CM-1. Based on the distribution of the CM-1.4 and M1a allotypes in inbred lines of chickens, 3 alleles are defined at the CM-1 locus.
通过用沙门氏菌O抗沙门氏菌O（UCD 3）凝集物免疫原鸡，产生了一种识别鸡17S Ig上遗传标记（CM-1.4）的同种抗血清。基于它们在放射免疫分析中抑制同种抗体（抗CM-1.4或抗M1a）与UCD 3 125I-17S Ig结合的能力，CM-1.4和M1a特异性存在于7S亚基和重链上。CM-1.4似乎在7S亚基上完全表达，但在重链上发生了改变。相比之下，抗M1a与7S亚基和重链的结合亲和力均降低，表明两种制剂中的M1a特异性均发生了改变。由于CM-1.4和M1a存在于17S Ig重链上，而不存在于7S Ig上，它们可能代表17S Ig重链恒定区的同种异型标记。在为重链指定了两种17S Ig同种异型特异性后，我们建议将控制它们表达的基因座称为CM-1。根据CM-1.4和M1a同种异型在鸡近交系中的分布，在CM-1基因座定义了3个等位基因。
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