脂蛋白脂肪酶在体外脂解过程中从人极低密度脂蛋白中去除的载脂蛋白C的再分布。

Redistribution of apolipoproteins C removed from human very low density lipoprotein during in vitro lipolysis by lipoprotein lipase.

作者信息

Glangeaud-Freudenthal M C, Burdin J, Ayrault-Jarrier M, Polonovski J

出版信息

Biochimie. 1981 Jun;63(6):485-94. doi: 10.1016/s0300-9084(81)80081-8.

Abstract

In order to localize the labelled apolipoproteins C removed from Very Low Density Lipoproteins (VLDL) during lipolysis by lipoprotein lipase, we used human VLDL labelled with 125I-labelled apolipoproteins C and employed density gradient ultracentrifugation to analyze lipolytic products. Triacylglycerol hydrolysis occurs when albumin is present even in the absence of serum or High Density Lipoproteins (HDL). In this case, apolipoproteins C were found to be located in several fractions, in different density regions. When either HDL or serum were present in the incubation medium, the removed apolipoproteins C were recovered in only one main fraction in the high density region (1.10 g/ml). Incubations in the presence of either HDL2 or HDL3 gave quite similar results.

摘要

为了定位在脂蛋白脂肪酶介导的脂解过程中从极低密度脂蛋白（VLDL）中去除的标记载脂蛋白C，我们使用用125I标记的载脂蛋白C标记的人VLDL，并采用密度梯度超速离心法分析脂解产物。即使在没有血清或高密度脂蛋白（HDL）的情况下，当存在白蛋白时也会发生三酰甘油水解。在这种情况下，发现载脂蛋白C位于几个不同密度区域的组分中。当孵育介质中存在HDL或血清时，去除的载脂蛋白C仅在高密度区域（1.10 g/ml）的一个主要组分中回收。在HDL2或HDL3存在下的孵育给出了非常相似的结果。