Parameswaran M, Arinze I J
Biol Neonate. 1981;39(5-6):260-5. doi: 10.1159/000241446.
Gluconeogenesis from (U-14C)-lactate occurred in hepatocytes prepared from term fetuses which lack cytosolic phosphoenolpyruvate carboxykinase and was almost completely inhibited by 3-mercaptopicolinate but was relatively insensitive to amino-oxyacetate. 12 h after birth when up to 32% of the total hepatic phosphoenolpyruvate carboxykinase activity was detectable in the cytosol, glucose synthesis was increased 4.4-fold in hepatocytes from fasted neonates and was partially (37%) sensitive to amino-oxyacetate. In livers of fasted 24-hour-old neonates total phosphoenolpyruvate carboxykinase activity was distributed between the mitochondria and the cytosol in the ratio of 60:40. In hepatocytes prepared from such animals, amino-oxyacetate inhibited glucose synthesis by about 56%, suggesting that up to half of the carbon flow from lactate to glucose was via the formation of phosphoenolpyruvate in the mitochondria. These studies indicate an important role for mitochondrial phosphoenolpyruvate carboxykinase in neonatal gluconeogenesis.
在缺乏胞质磷酸烯醇式丙酮酸羧激酶的足月胎儿制备的肝细胞中，由（U-14C）-乳酸进行的糖异生发生了，并且几乎完全被3-巯基吡啶甲酸盐抑制，但对氨基氧乙酸相对不敏感。出生后12小时，当胞质中可检测到高达32%的总肝磷酸烯醇式丙酮酸羧激酶活性时，禁食新生儿的肝细胞中葡萄糖合成增加了4.4倍，并且对氨基氧乙酸部分敏感（37%）。在禁食24小时的新生儿肝脏中，总磷酸烯醇式丙酮酸羧激酶活性在线粒体和胞质之间以60:40的比例分布。在由此类动物制备的肝细胞中，氨基氧乙酸抑制葡萄糖合成约56%，这表明从乳酸到葡萄糖的碳流中高达一半是通过线粒体中磷酸烯醇式丙酮酸的形成来实现的。这些研究表明线粒体磷酸烯醇式丙酮酸羧激酶在新生儿糖异生中起重要作用。
