醋酸锌作为不稳定血红蛋白的沉淀剂。
Zinc acetate as a precipitant of unstable haemoglobins.
作者信息
Carrell R W, Lehmann H
出版信息
J Clin Pathol. 1981 Jul;34(7):796-9. doi: 10.1136/jcp.34.7.796.
Abstract
Whereas the addition of four moles of zinc acetate to one of normal human haemoglobin at pH 7.4 results in a clear solution, addition to unstable haemoglobin results in precipitate formation. Within certain limits no false-positive results are obtained with methaemoglobin, and with fetal haemoglobin. With excess zinc, normal haemoglobin also precipitates, but this process is reversible and can be used for a rapid concentration of dilute solutions of normal haemoglobin.
摘要
在pH 7.4的条件下，向一摩尔正常人血红蛋白中加入四摩尔醋酸锌会得到澄清溶液，而加入到不稳定血红蛋白中则会形成沉淀。在一定限度内，高铁血红蛋白和胎儿血红蛋白不会产生假阳性结果。锌过量时，正常血红蛋白也会沉淀，但这个过程是可逆的，可用于快速浓缩正常血红蛋白的稀溶液。
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本文引用的文献
1
Factors influencing the formation of Robison ester.
Biochem J. 1939 Aug;33(8):1241-4. doi: 10.1042/bj0331241.
2
THE BINDING OF CUPRIC AND ZINC IONS TO CRYSTALLINE SPERM WHALE MYOGLOBIN.
J Mol Biol. 1965 May;12:130-7. doi: 10.1016/s0022-2836(65)80287-x.
3
Haemoglobin Hammersmith (beta-42 (CDI) Phe replaced by ser).
Nature. 1967 Nov 18;216(5116):663-5. doi: 10.1038/216663a0.
4
Köln haemoglobinopathy. Further data and a comparison with other hereditary Heinz body anaemias.
Br J Haematol. 1967 May;13(3):394-408. doi: 10.1111/j.1365-2141.1967.tb08754.x.
5
Effect of zinc on increasing oxygen affinity of sickle and normal red blood cells.
Biochem Biophys Res Commun. 1973 Jul 17;53(2):560-6. doi: 10.1016/0006-291x(73)90698-0.
6
A simple method for the detection of unstable haemoglobins.
Br J Haematol. 1972 Nov;23(5):615-9. doi: 10.1111/j.1365-2141.1972.tb07096.x.
7
Studies on the interaction of zinc with human hemoglobin.
Arch Biochem Biophys. 1974 Aug;163(2):742-8. doi: 10.1016/0003-9861(74)90536-0.
8
Double heterozygosity for two unstable haemoglobins: Hb Sydney (beta67[E11] Val leads to Ala) and Hb Coventry (beta141[H19] Leu deleted).
Br J Haematol. 1978 Feb;38(2):195-209. doi: 10.1111/j.1365-2141.1978.tb01036.x.
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