通过用二酰胺或膜流动性剂A2C形成帽状物来界定的细胞膜受体类别。

Cell membrane receptor classes delimited through cap formation either with diamide or with membrane mobility agent, A2C.

作者信息

Kosower N S, Faltin Z, Kosower E M

出版信息

J Immunol Methods. 1981;41(2):215-23. doi: 10.1016/0022-1759(81)90244-1.

Abstract

Receptors on normal human peripheral blood lymphocytes can be divided into two classes by means of the capping response exhibited in the presence of the reagents, diamide or colchicine (microtubule-related) and A2C (microtubule-independent). Diamide and colchicine promote capping of concanavalin A (Con A) receptors. Diamide capping is reversible, while colchicine capping is not reversible under the conditions used. A2C does not promote the capping of Con A receptors. In contrast, diamide and colchicine do not affect the rate at which either anti-immunoglobulin (anti-Ig) or wheat germ agglutinin (WGA) receptors cap, but A2C effectively enhances cap formation for both anti-Ig and WGA receptors. The simplicity of the classification method promises to be of use in the investigation of membrane receptors.

摘要

通过在存在试剂二酰胺或秋水仙碱（与微管相关）和A2C（与微管无关）的情况下表现出的封帽反应，可将正常人外周血淋巴细胞上的受体分为两类。二酰胺和秋水仙碱促进伴刀豆球蛋白A（Con A）受体的封帽。在所用条件下，二酰胺封帽是可逆的，而秋水仙碱封帽是不可逆的。A2C不促进Con A受体的封帽。相反，二酰胺和秋水仙碱不影响抗免疫球蛋白（抗Ig）或麦胚凝集素（WGA）受体封帽的速率，但A2C可有效增强抗Ig和WGA受体的封帽形成。这种分类方法的简便性有望用于膜受体的研究。