Rychlik W, Zagórski W
Acta Biochim Pol. 1978;25(2):129-46.
Optimum conditions for translation of eukaryotic, prokaryotic and synthetic templates in wheat germ cell-free extract were determined. 1. Translation of eukaryotic message (BMV RNA and TMV RNA) was at optimum at the same concentrations of K+, Mg(2+), HEPES and spermine. In optimal conditions the efficiency of translation was high, for BMV RNA being equal to 220 pmoles and for TMV RNA, to 280 pmoles of leucine incorporated per 1 microgram of template. Prokaryotic template (Qbeta RNA) was translated under different ionic conditions. 2. Translation of synthetic template [poly(U)] was at optimum at fairly higher concentrations of K+ and Mg(2+) than those optimal for natural template translation. 3. Efficient translation of natural and synthetic templates depends on complete removal of inhibitors found in the wheat germ cell-free extract. Action of these inhibitors could be mimicked by adenine nucleotides.
确定了在小麦胚无细胞提取物中翻译真核、原核和合成模板的最佳条件。1. 真核信使（BMV RNA和TMV RNA）的翻译在相同浓度的K⁺、Mg²⁺、HEPES和精胺下达到最佳。在最佳条件下，翻译效率很高，对于BMV RNA，每1微克模板掺入的亮氨酸为220皮摩尔，对于TMV RNA，为280皮摩尔。原核模板（Qβ RNA）在不同的离子条件下进行翻译。2. 合成模板[聚（U）]的翻译在比天然模板翻译最佳浓度相当高的K⁺和Mg²⁺浓度下达到最佳。3. 天然和合成模板的有效翻译取决于从小麦胚无细胞提取物中完全去除抑制剂。这些抑制剂的作用可以被腺嘌呤核苷酸模拟。
