Marie J P, Izaguirre C A, Civin C I, Mirro J, McCulloch E A
Blood. 1981 Oct;58(4):708-11.
The continuous cell line K-562, derived from a patient with CML in blast crisis, was examined for markers of granulopoietic (My-1) and erythropoietic (spectrin) differentiation, using specific antibodies detected by indirect immunofluorescence. Both markers were seen, and in 10%--30% of cells, both were present in the same cells. In contrast, the continuous leukemic line HL-60 and KGI contained My-1 only. Controls consisted of colonies in culture containing both granulopoietic and erythropoietic cells (CFU-GEMM). In these, My-1 was seen only in granulopoietic cells and spectrin in erythropoietic cells. The suggestion is advanced that genes coding for differentiation markers are expressed abnormally in K-562.
利用间接免疫荧光检测的特异性抗体，对源自一名处于急变期慢性粒细胞白血病患者的连续细胞系K - 562进行了粒系造血（髓过氧化物酶 - 1，My - 1）和红系造血（血影蛋白）分化标志物的检测。两种标志物均可见，并且在10% - 30%的细胞中，两种标志物存在于同一细胞中。相比之下，连续白血病细胞系HL - 60和KGI仅含有My - 1。对照包括含有粒系造血细胞和红系造血细胞的培养集落（粒 - 红 - 巨核 - 巨噬细胞集落形成单位，CFU - GEMM）。在这些对照中，My - 1仅在粒系造血细胞中可见，而血影蛋白在红系造血细胞中可见。有人提出，编码分化标志物的基因在K - 562中异常表达。
