Filippovich I I, Poliakova G N, Gostimskiĭ S A
Biokhimiia. 1981 Oct;46(10):1787-94.
The morphological properties and polypeptide composition of inner chloroplast membranes of normal and mutant pea plants (nuclear mutant "Chlorophyll I") were compared. The total fractions of chloroplast membranes from both sources were separated in a stepwise sucrose density gradient resulting in two fractions. The major fraction from mutant plants, unlike that from normal plants, does not contain thylakoids of the grana but is represented by reticular and cellulate structures, in which each cell corresponds to a thylakoid of macrograna and whose size exceeds that of normal 3 - 4-fold. In addition the inner membranes of mutant plant chloroplasts do not contain membrane polypeptides with molecular weight of 32 000 and 35 000.
对正常和突变豌豆植株(核突变体“叶绿素 I”)叶绿体内膜的形态特性和多肽组成进行了比较。将来自这两种来源的叶绿体膜总组分在逐步蔗糖密度梯度中进行分离,得到两个组分。与正常植株不同,突变植株的主要组分不含基粒类囊体,而是由网状和蜂窝状结构组成,其中每个单元对应一个大基粒类囊体,其大小比正常的大 3 - 4 倍。此外,突变植株叶绿体的内膜不含分子量为 32000 和 35000 的膜多肽。