新生大鼠膈肌对β-银环蛇毒素的反应减弱。
Diminished response to beta-bungarotoxin in neonatal rat diaphragm.
作者信息
Gundersen C B
出版信息
J Physiol. 1981 May;314:151-9. doi: 10.1113/jphysiol.1981.sp013697.
Abstract
- The effects of beta-bungarotoxin on acetylcholine turnover were studied in neonatal rat diaphragms using a gas chromatographic mass spectrometric assay to measure acetylcholine and choline. 2. Injection of neonatal rats (1-15 days old) with a fixed dose of beta-bungarotoxin resulted in a shorter time to death in the older animals. 3. This increased lethal potency of beta-bungarotoxin in the older rats was correlated with an enhancement of the stimulatory effect of the toxin on acetylcholine synthesis in diaphragm in vivo and in vitro. 4. The reduced effect of beta-bungarotoxin in promoting the accumulation of acetylcholine in new-born rat diaphragms was not due to an enhancement by the toxin of acetylcholine output from these preparations. 5. These results demonstrate that the stimulatory effect of beta-bungarotoxin on acetylcholine synthesis in diaphragm is a function of the age of the rat. Thus, beta-bungarotoxin may be valuable as a probe of the maturation of presynaptic function in rat skeletal muscle.
摘要
- 使用气相色谱质谱分析法测量乙酰胆碱和胆碱，研究了β-银环蛇毒素对新生大鼠膈肌乙酰胆碱周转的影响。2. 给新生大鼠（1至15日龄）注射固定剂量的β-银环蛇毒素，结果发现年龄较大的动物死亡时间更短。3. β-银环蛇毒素在老年大鼠中这种增加的致死效力与该毒素在体内和体外对膈肌乙酰胆碱合成的刺激作用增强相关。4. β-银环蛇毒素促进新生大鼠膈肌中乙酰胆碱积累的作用减弱，并非由于该毒素增强了这些制剂中乙酰胆碱的释放。5. 这些结果表明，β-银环蛇毒素对膈肌乙酰胆碱合成的刺激作用是大鼠年龄的函数。因此，β-银环蛇毒素作为大鼠骨骼肌突触前功能成熟的探针可能具有重要价值。
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本文引用的文献
1
A histochemical study of cholinesterase during formation of the motor end plate of the albino rat.
J Exp Zool. 1951 Apr;116(3):397-413. doi: 10.1002/jez.1401160303.
2
BIOCHEMISTRY OF THE AVIAN CENTRAL NERVOUS SYSTEM . II. 5-HYDROXYTRYPTAMINE, ACETYLCHOLINE, 3,4-DIHYDROXYPHENYLETHYLAMINE, AND NOREPINEPHRINE IN SEVERAL DISCRETE AREAS OF THE PIGEON BRAIN.
J Neurochem. 1965 Mar;12:221-30. doi: 10.1111/j.1471-4159.1965.tb06758.x.
3
ISOLATION OF NEUROTOXINS FROM THE VENOM OF BUNGARUS MULTICINCTUS AND THEIR MODES OF NEUROMUSCULAR BLOCKING ACTION.
Arch Int Pharmacodyn Ther. 1963 Jul 1;144:241-57.
4
A study of foetal and new-born rat muscle fibres.
J Physiol. 1962 Aug;162(3):393-408. doi: 10.1113/jphysiol.1962.sp006941.
5
beta-Bungarotoxin elevates diaphragm acetylcholine levels.
Brain Res. 1980 Jan 27;182(2):486-90. doi: 10.1016/0006-8993(80)91210-x.
6
Beta-bungarotoxin stimulates the synthesis and accumulation of acetylcholine in rat phrenic nerve diaphragm preparations.
J Physiol. 1981 Jan;310:13-35. doi: 10.1113/jphysiol.1981.sp013535.
7
Neuromuscular transmission in new-born rats.
J Physiol. 1970 Aug;209(3):701-9. doi: 10.1113/jphysiol.1970.sp009187.
8
Synthesis, storage and release of [14C]acetylcholine in isolated rat diaphragm muscles.
J Physiol. 1970 Jan;206(1):145-66. doi: 10.1113/jphysiol.1970.sp009003.
9
Choline acetyltransferase activity in rat skeletal muscles during postnatal development.
Exp Neurol. 1972 Aug;36(2):378-88. doi: 10.1016/0014-4886(72)90031-3.
10
The relationship of choline acetyltransferase activity at the neuromuscular junction to changes in muscle mass and function.
J Physiol. 1974 Jan;236(1):247-57. doi: 10.1113/jphysiol.1974.sp010433.
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