Comín E J, Elías M M, Grossman M, Rodriguez Garay E A
Res Commun Chem Pathol Pharmacol. 1981 Oct;34(1):169-72.
The effect produced by unconjugated bilirubin (UB) on p-aminohippuric acid (PAH) translocation from plasma to the urine, was analyzed by using an isolated rat kidney preparation applying the multiple indicator dilution technique. Arterial -to- urine mean transit times and fractional recoveries of creatinine and PAH in the venous effluent, were calculated before and after the incorporation of UB into the system. PAH influx into cells, its efflux from the cells to the capillaries, and its movement between lumen and tubular cells were also calculated. The results indicated that PAH influx into cells was impaired in the presence of UB. The phenomenom was shown to be dose-dependent. On the contrary, the efflux of PAH to the capillaries that was also impaired by UB, seemed to be unrelated to the pigment concentration within the system. Movement of PAH between lumen and tubular cells also appeared to be affected by UB. The results obtained suggested that UB interferes with the renal handling of PAH but the mechanism involved could not be clarified with the model used.
采用多指示剂稀释技术，利用离体大鼠肾脏标本，分析了非结合胆红素（UB）对对氨基马尿酸（PAH）从血浆转运至尿液的影响。在将UB加入系统前后，计算了动脉到尿液的平均转运时间以及静脉流出液中肌酐和PAH的分数回收率。还计算了PAH进入细胞的流量、从细胞到毛细血管的流出量以及在管腔和肾小管细胞之间的移动情况。结果表明，在存在UB的情况下，PAH进入细胞的过程受到损害。这种现象呈剂量依赖性。相反，PAH向毛细血管的流出也受到UB的损害，这似乎与系统内色素浓度无关。PAH在管腔和肾小管细胞之间的移动似乎也受到UB的影响。所得结果表明，UB干扰了肾脏对PAH的处理，但所使用的模型无法阐明其中涉及的机制。
