Knight L C, Budzynski A Z, Olexa S A
Thromb Haemost. 1981 Oct;46(3):593-6.
The properties of human fibrinogen labeled with 125-Iodine using Iodogen (1, 3, 4, 6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril) as an oxidizing agent were compared with those of an iodine monochloride labeled counterpart. It was found that thrombin clottability, binding to staphylococci, the relative specific radioactivity of the A alpha, B beta, and gamma chains and in vivo clearance from plasma in rabbits were the same in these two labeled fibrinogen preparations. Labeling efficiency was higher when iodogen was used. It is concluded that human fibrinogen labeled with radioiodine using the Iodogen technique is suitable for studies in vitro and in vivo.
以碘代甘脲（1, 3, 4, 6 - 四氯 - 3α, 6α - 二苯基甘脲）作为氧化剂标记的125碘人纤维蛋白原的特性，与一氯化碘标记的对应物的特性进行了比较。结果发现，在这两种标记的纤维蛋白原制剂中，凝血酶凝块形成能力、与葡萄球菌的结合、Aα、Bβ和γ链的相对比放射性以及在兔体内从血浆中的清除情况均相同。使用碘代甘脲时标记效率更高。得出的结论是，采用碘代甘脲技术用放射性碘标记的人纤维蛋白原适用于体内和体外研究。
